Images
自动图像算法处理
如果在启动 SAW count
或 SAW realign
分析时加入了图像数据,图像相关的结果文件将被保存在/outs/image
目录下,具体内容取决于图像 QC 结果和手动处理信息。
File | Discription |
---|---|
*_regist.tif | The panoramic image, having been registered with the raw expression matrix, is from staining layers in TIFF format. |
*_tissue_cut.tif | Tissue segmentation result of the registered panoramic image. |
*_mask.tif | Cell segmentation result of the registered panoramic image. |
*_mask_edm_dis_10.tif | The result of the cell border expanding is based on the cell segmentation image. *_mask.tif . dis_10 represents the expansion of 10 pixels. |
对于每个图像文件类型,这里以一个小鼠全脑样本为例进行展示:

Registered panoramic image

Tissue segementation in purple

Cell segmentation

Adjusted cell segmentation
无图流程
如果在启动 SAW count
或 SAW realign
分析时没有加入图像数据,或者图像 QC 结果为失败,分析流程会基于表达矩阵进行组织分割。
File | Discription |
---|---|
bin1_img_tissue_cut.tif | Tissue segmentation result of the expression data. |
这里以一个小鼠小肠样片为例进行展示:
.jpg)
Tissue segementation based on expression data