Images

自动图像算法处理

如果在启动 SAW countSAW realign 分析时加入了图像数据,图像相关的结果文件将被保存在/outs/image 目录下,具体内容取决于图像 QC 结果和手动处理信息。

FileDiscription
*_regist.tifThe panoramic image, having been registered with the raw expression matrix, is from staining layers in TIFF format.
*_tissue_cut.tifTissue segmentation result of the registered panoramic image.
*_mask.tifCell segmentation result of the registered panoramic image.
*_mask_edm_dis_10.tifThe result of the cell border expanding is based on the cell segmentation image. *_mask.tif. dis_10 represents the expansion of 10 pixels.

对于每个图像文件类型,这里以一个小鼠全脑样本为例进行展示:

Registered panoramic image

Tissue segementation in purple

Cell segmentation

Adjusted cell segmentation

无图流程

如果在启动 SAW countSAW realign 分析时没有加入图像数据,或者图像 QC 结果为失败,分析流程会基于表达矩阵进行组织分割。

FileDiscription
bin1_img_tissue_cut.tifTissue segmentation result of the expression data.

这里以一个小鼠小肠样片为例进行展示:

Heatmaop of expression matrix

Tissue segementation based on expression data

© 2025 STOmics Tech. All rights reserved.Modified: 2025-03-07 10:28:04

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